At each concentration, 10 experimental animals of 48 hour s old Artemia salina Leach larvae were used. The BSLT method used consisted of 6 concentration treatments, namely 20 ppm, 60 ppm, 100 ppm, 140 ppm, 180 ppm, and 0 ppm a negative control, each of which was repeated three times. The purpose of this study was to determine the percentage of mortality data and the LC50 value of tapak dara methanol extract (Catharanthus roseus) against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method. Tapak dara (Catharanthus roseus) is a plant belonging to the Apocynaceae family originating from Central America and is generally grown as an ornamental plant.
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